加速细胞株开发的一些新进展

Lauren Tanabe June 27 2019Biocompare

亿? Susan Sharfstein

 

CHOSharfsteinFDACHO CHO齿:CHO

 

ORF ,openreading frame)DNA

 

SolentimIan Taylor2019SolentimATUMATUMLeapIn Transposase®TaylorGOIGene of interest200

 

SolentimInstiGRO

 

CHOs

 

SharfsteinCHOSharfstein:UCB Celltech10

 

 

(GS)(DHFR)GSGOIGSGSGSnull CHO K1GS

 

CHOCRISPRHocusLocusGOI3 siRNAmRNAsiRNAGOI/siRNAhttp://hocuslocus.com/applications/

siRNAmRNAmRNACD4siRNA siRNACD4mRNACD4CD4siRNACD4

Sharfstein

 

CRISPR:使

 

SharfsteinCharles RiverAnneMarie ZuurmondCRISPR RNACRISPRmRNA宿

 

CRISPR沿Zuurmond:Cas9likeCRISPRCas14Cas9DNAssDNACas9PAM

 

CRISPR/CasCRISPRCRISPR/CasCRISPR

 PAMCas使spCas9NGGNspCas9PAM广CasDNANGG

 Broad InsititudeDavid LiuCasxCas9使广spCas9xCas9广PAM

 

 

ZuurmondCRISPRCharles River使CRISPRorthogonal approachCRISPRrAAV()CRISPRproof of concept使

 

Zummond:CRISPRCRISPR

 

 

TaylorFDA广

 

SolentimVIPS(Verified Insitu Plate Seeding)便VIPS96SolentimCell MetricTaylorFDA

 

,CRISPR,,, ()