加速细胞株开发的一些新进展

Lauren Tanabe June 27 2019Biocompare 亿? Susan Sharfstein   CHOSharfsteinFDACHO CHO齿:CHO   ORF ,openreading frame)DNA   SolentimIan Taylor2019SolentimATUMATUMLeapIn Transposase®TaylorGOIGene of interest200   SolentimInstiGRO   CHOs   SharfsteinCHOSharfstein:UCB Celltech10     (GS)(DHFR)GSGOIGSGSGSnull CHO K1GS   CHOCRISPRHocusLocusGOI3 siRNAmRNAsiRNAGOI/siRNAhttp://hocuslocus.com/applications/ siRNAmRNAmRNACD4siRNA siRNACD4mRNACD4CD4siRNACD4 Sharfstein   CRISPR:使   SharfsteinCharles RiverAnneMarie ZuurmondCRISPR RNACRISPRmRNA宿   CRISPR沿Zuurmond:Cas9likeCRISPRCas14Cas9DNAssDNACas9PAM   CRISPR/CasCRISPRCRISPR/CasCRISPR  PAMCas使spCas9NGGNspCas9PAM广CasDNANGG  Broad InsititudeDavid LiuCasxCas9使广spCas9xCas9广PAM     ZuurmondCRISPRCharles River使CRISPRorthogonal approachCRISPRrAAV()CRISPRproof of concept使   Zummond:CRISPRCRISPR     TaylorFDA广   SolentimVIPS(Verified Insitu Plate Seeding)便VIPS96SolentimCell MetricTaylorFDA   ,CRISPR,,, ()