利用Axion Maestro研究神经类器官

Axion Maestro

OrganoidMaestro

Hear the sound of symphonies

201967OrganoidsApproximating organs

Science Vol 364, Issue 6444 07 June 2019

66线2116RNA

95%BroadStanley

1

Silvia Velasco et al., (2019) Individual brain organoids reproducibly form cell diversity of the human cerebral cortex. Nature. Doi: 10.1038/s415860191289x

Axion Maestro

Axion Biosystems

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3D

Axion BioSystemsMaestro ProEdgeMaestro

()()Maestro MEAactivitysynchronyoscillation

Oscillationspikes

(hiPSCs)Maestro(A)30network bursts(BC)

A)4

B)

C)B16spikesbursts绿

(SFEBs)hiPSCsMEAsSFEBs3090SFEBs

A)MEASFEBPhillips, A. W., Nestor, J. E., &Nestor, M. W. (2017). Developing HiPSC Derived Serum Free Embryoid Bodies forthe Interrogation of 3D Stem Cell Cultures Using Physiologically RelevantAssays. J Vis Exp(125). doi:10.3791/55799.

B)firing rateSFEB3090

 

 

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Neural Organoid Activity Assay